diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 8d9a956e..8c22faaa 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -19,6 +19,13 @@ repos:
       - id: pyupgrade
         args: [--py39-plus]
 
+  # Automatically sort python imports
+  - repo: https://github.com/pycqa/isort
+    rev: 5.10.1
+    hooks:
+      - id: isort
+        args: [--profile, black]
+
   # Autoformat: Python code
   - repo: https://github.com/psf/black
     rev: 22.1.0
@@ -53,9 +60,10 @@ repos:
   - repo: https://github.com/pre-commit/pre-commit-hooks
     rev: v4.1.0
     hooks:
-      - id: trailing-whitespace
-      - id: end-of-file-fixer
       - id: check-added-large-files
+      - id: end-of-file-fixer
+      - id: requirements-txt-fixer
+      - id: trailing-whitespace
 
   # Lint: Dockerfile
   - repo: https://github.com/hadolint/hadolint.git
@@ -108,12 +116,13 @@ repos:
       - id: nbstripout
 
   # nbQA provides tools from the Python ecosystem like
-  # pyupgrade, black, and flake8, adjusted for notebooks.
+  # pyupgrade, isort, black, and flake8, adjusted for notebooks.
   - repo: https://github.com/nbQA-dev/nbQA
     rev: 1.3.1
     hooks:
       - id: nbqa-pyupgrade
         args: [--py39-plus]
+      - id: nbqa-isort
       - id: nbqa-black
         args: [--target-version=py39]
       - id: nbqa-flake8
diff --git a/base-notebook/jupyter_server_config.py b/base-notebook/jupyter_server_config.py
index c5f70c6f..ef0380bd 100644
--- a/base-notebook/jupyter_server_config.py
+++ b/base-notebook/jupyter_server_config.py
@@ -1,11 +1,11 @@
 # Copyright (c) Jupyter Development Team.
 # Distributed under the terms of the Modified BSD License.
 # mypy: ignore-errors
-from jupyter_core.paths import jupyter_data_dir
-import subprocess
 import os
 import stat
+import subprocess
 
+from jupyter_core.paths import jupyter_data_dir
 
 c = get_config()  # noqa: F821
 c.ServerApp.ip = "0.0.0.0"
diff --git a/tagging/create_manifests.py b/tagging/create_manifests.py
index 5704801c..c784bdf5 100755
--- a/tagging/create_manifests.py
+++ b/tagging/create_manifests.py
@@ -5,13 +5,14 @@ import argparse
 import datetime
 import logging
 import os
+
 from docker.models.containers import Container
+
 from .docker_runner import DockerRunner
 from .get_taggers_and_manifests import get_taggers_and_manifests
 from .git_helper import GitHelper
 from .manifests import ManifestHeader, ManifestInterface
 
-
 LOGGER = logging.getLogger(__name__)
 
 
diff --git a/tagging/docker_runner.py b/tagging/docker_runner.py
index 08a93867..9d71f27b 100644
--- a/tagging/docker_runner.py
+++ b/tagging/docker_runner.py
@@ -1,11 +1,11 @@
 # Copyright (c) Jupyter Development Team.
 # Distributed under the terms of the Modified BSD License.
-from typing import Optional
+import logging
 from types import TracebackType
+from typing import Optional
+
 import docker
 from docker.models.containers import Container
-import logging
-
 
 LOGGER = logging.getLogger(__name__)
 
diff --git a/tagging/get_taggers_and_manifests.py b/tagging/get_taggers_and_manifests.py
index d8730a32..2a882c1b 100644
--- a/tagging/get_taggers_and_manifests.py
+++ b/tagging/get_taggers_and_manifests.py
@@ -1,6 +1,7 @@
 # Copyright (c) Jupyter Development Team.
 # Distributed under the terms of the Modified BSD License.
 from typing import Optional
+
 from .images_hierarchy import ALL_IMAGES
 from .manifests import ManifestInterface
 from .taggers import TaggerInterface
diff --git a/tagging/images_hierarchy.py b/tagging/images_hierarchy.py
index 690ca121..9a68f97e 100644
--- a/tagging/images_hierarchy.py
+++ b/tagging/images_hierarchy.py
@@ -2,29 +2,30 @@
 # Distributed under the terms of the Modified BSD License.
 from dataclasses import dataclass, field
 from typing import Optional
+
+from .manifests import (
+    AptPackagesManifest,
+    CondaEnvironmentManifest,
+    JuliaPackagesManifest,
+    ManifestInterface,
+    RPackagesManifest,
+    SparkInfoManifest,
+)
 from .taggers import (
-    TaggerInterface,
-    SHATagger,
     DateTagger,
-    UbuntuVersionTagger,
-    PythonVersionTagger,
-    JupyterNotebookVersionTagger,
-    JupyterLabVersionTagger,
-    JupyterHubVersionTagger,
-    RVersionTagger,
-    TensorflowVersionTagger,
-    JuliaVersionTagger,
-    SparkVersionTagger,
     HadoopVersionTagger,
     JavaVersionTagger,
-)
-from .manifests import (
-    ManifestInterface,
-    CondaEnvironmentManifest,
-    AptPackagesManifest,
-    RPackagesManifest,
-    JuliaPackagesManifest,
-    SparkInfoManifest,
+    JuliaVersionTagger,
+    JupyterHubVersionTagger,
+    JupyterLabVersionTagger,
+    JupyterNotebookVersionTagger,
+    PythonVersionTagger,
+    RVersionTagger,
+    SHATagger,
+    SparkVersionTagger,
+    TaggerInterface,
+    TensorflowVersionTagger,
+    UbuntuVersionTagger,
 )
 
 
diff --git a/tagging/manifests.py b/tagging/manifests.py
index c0bceaf6..e7a51924 100644
--- a/tagging/manifests.py
+++ b/tagging/manifests.py
@@ -1,7 +1,8 @@
 # Copyright (c) Jupyter Development Team.
 # Distributed under the terms of the Modified BSD License.
-from plumbum.cmd import docker
 from docker.models.containers import Container
+from plumbum.cmd import docker
+
 from .docker_runner import DockerRunner
 from .git_helper import GitHelper
 
diff --git a/tagging/tag_image.py b/tagging/tag_image.py
index 364be059..c1783f52 100755
--- a/tagging/tag_image.py
+++ b/tagging/tag_image.py
@@ -3,12 +3,13 @@
 # Distributed under the terms of the Modified BSD License.
 import argparse
 import logging
+
 from plumbum.cmd import docker
+
 from .docker_runner import DockerRunner
 from .get_taggers_and_manifests import get_taggers_and_manifests
 from .github_set_env import github_set_env
 
-
 LOGGER = logging.getLogger(__name__)
 
 
diff --git a/tagging/taggers.py b/tagging/taggers.py
index 09f017ac..a39334f5 100644
--- a/tagging/taggers.py
+++ b/tagging/taggers.py
@@ -1,9 +1,11 @@
 # Copyright (c) Jupyter Development Team.
 # Distributed under the terms of the Modified BSD License.
 from datetime import datetime
+
 from docker.models.containers import Container
-from .git_helper import GitHelper
+
 from .docker_runner import DockerRunner
+from .git_helper import GitHelper
 
 
 def _get_program_version(container: Container, program: str) -> str:
diff --git a/tests/all-spark-notebook/test_spark_notebooks.py b/tests/all-spark-notebook/test_spark_notebooks.py
index 9d2475b3..ee64dfb2 100644
--- a/tests/all-spark-notebook/test_spark_notebooks.py
+++ b/tests/all-spark-notebook/test_spark_notebooks.py
@@ -2,10 +2,9 @@
 # Distributed under the terms of the Modified BSD License.
 
 import logging
-
-import pytest  # type: ignore
 from pathlib import Path
 
+import pytest  # type: ignore
 from conftest import TrackedContainer
 
 LOGGER = logging.getLogger(__name__)
diff --git a/tests/base-notebook/test_container_options.py b/tests/base-notebook/test_container_options.py
index de1261b3..03405694 100644
--- a/tests/base-notebook/test_container_options.py
+++ b/tests/base-notebook/test_container_options.py
@@ -1,12 +1,11 @@
 # Copyright (c) Jupyter Development Team.
 # Distributed under the terms of the Modified BSD License.
+import logging
 import pathlib
 import time
-import logging
 
 import pytest  # type: ignore
 import requests
-
 from conftest import TrackedContainer, find_free_port
 
 LOGGER = logging.getLogger(__name__)
diff --git a/tests/base-notebook/test_outdated.py b/tests/base-notebook/test_outdated.py
index ac13da56..26416545 100644
--- a/tests/base-notebook/test_outdated.py
+++ b/tests/base-notebook/test_outdated.py
@@ -5,7 +5,6 @@ import logging
 
 import pytest  # type: ignore
 from conftest import TrackedContainer
-
 from package_helper import CondaPackageHelper
 
 LOGGER = logging.getLogger(__name__)
diff --git a/tests/base-notebook/test_package_managers.py b/tests/base-notebook/test_package_managers.py
index 9f36185a..d613bf3c 100644
--- a/tests/base-notebook/test_package_managers.py
+++ b/tests/base-notebook/test_package_managers.py
@@ -2,8 +2,8 @@
 # Distributed under the terms of the Modified BSD License.
 
 import logging
-import pytest  # type: ignore
 
+import pytest  # type: ignore
 from conftest import TrackedContainer
 
 LOGGER = logging.getLogger(__name__)
diff --git a/tests/base-notebook/test_packages.py b/tests/base-notebook/test_packages.py
index ddee10ce..ea1e9bb4 100644
--- a/tests/base-notebook/test_packages.py
+++ b/tests/base-notebook/test_packages.py
@@ -38,11 +38,10 @@ Example:
 """
 
 import logging
+from typing import Callable, Iterable
 
 import pytest  # type: ignore
 from conftest import TrackedContainer
-from typing import Callable, Iterable
-
 from package_helper import CondaPackageHelper
 
 LOGGER = logging.getLogger(__name__)
diff --git a/tests/base-notebook/test_python.py b/tests/base-notebook/test_python.py
index 27ca9a03..75db24ff 100644
--- a/tests/base-notebook/test_python.py
+++ b/tests/base-notebook/test_python.py
@@ -2,9 +2,8 @@
 # Distributed under the terms of the Modified BSD License.
 import logging
 
-from packaging import version  # type: ignore
-
 from conftest import TrackedContainer
+from packaging import version  # type: ignore
 
 LOGGER = logging.getLogger(__name__)
 
diff --git a/tests/base-notebook/test_start_container.py b/tests/base-notebook/test_start_container.py
index 0322db82..fffdf68f 100644
--- a/tests/base-notebook/test_start_container.py
+++ b/tests/base-notebook/test_start_container.py
@@ -2,11 +2,11 @@
 # Distributed under the terms of the Modified BSD License.
 
 import logging
+import time
 from typing import Optional
+
 import pytest  # type: ignore
 import requests
-import time
-
 from conftest import TrackedContainer, find_free_port
 
 LOGGER = logging.getLogger(__name__)
diff --git a/tests/conftest.py b/tests/conftest.py
index 18d7435f..fd4e4704 100644
--- a/tests/conftest.py
+++ b/tests/conftest.py
@@ -1,19 +1,17 @@
 # Copyright (c) Jupyter Development Team.
 # Distributed under the terms of the Modified BSD License.
-from contextlib import closing
-import os
 import logging
+import os
 import socket
+from contextlib import closing
 from typing import Any, Optional
 
 import docker
-from docker.models.containers import Container
 import pytest  # type: ignore
 import requests
-
-from urllib3.util.retry import Retry
+from docker.models.containers import Container
 from requests.adapters import HTTPAdapter
-
+from urllib3.util.retry import Retry
 
 LOGGER = logging.getLogger(__name__)
 
diff --git a/tests/minimal-notebook/data/notebook_math.ipynb b/tests/minimal-notebook/data/notebook_math.ipynb
index 951f751e..5b028b1d 100644
--- a/tests/minimal-notebook/data/notebook_math.ipynb
+++ b/tests/minimal-notebook/data/notebook_math.ipynb
@@ -27,7 +27,7 @@
    },
    "outputs": [],
    "source": [
-    "from sympy import init_printing, symbols, sin, diff, integrate"
+    "from sympy import diff, init_printing, integrate, sin, symbols"
    ]
   },
   {
diff --git a/tests/minimal-notebook/test_nbconvert.py b/tests/minimal-notebook/test_nbconvert.py
index 0763fb4a..3c70794b 100644
--- a/tests/minimal-notebook/test_nbconvert.py
+++ b/tests/minimal-notebook/test_nbconvert.py
@@ -2,10 +2,9 @@
 # Distributed under the terms of the Modified BSD License.
 
 import logging
-
-import pytest  # type: ignore
 from pathlib import Path
 
+import pytest  # type: ignore
 from conftest import TrackedContainer
 
 LOGGER = logging.getLogger(__name__)
diff --git a/tests/package_helper.py b/tests/package_helper.py
index 358f3951..88738ba5 100644
--- a/tests/package_helper.py
+++ b/tests/package_helper.py
@@ -22,17 +22,16 @@
 # OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
 # SOFTWARE.
 
+import json
+import logging
 import re
 from collections import defaultdict
 from itertools import chain
-import logging
-import json
 from typing import Any, Optional
-from docker.models.containers import Container
-
-from tabulate import tabulate
 
 from conftest import TrackedContainer
+from docker.models.containers import Container
+from tabulate import tabulate
 
 LOGGER = logging.getLogger(__name__)
 
diff --git a/tests/pyspark-notebook/test_spark.py b/tests/pyspark-notebook/test_spark.py
index dcb9fe6b..416fb099 100644
--- a/tests/pyspark-notebook/test_spark.py
+++ b/tests/pyspark-notebook/test_spark.py
@@ -4,7 +4,6 @@ import logging
 
 from conftest import TrackedContainer
 
-
 LOGGER = logging.getLogger(__name__)
 
 
diff --git a/tests/run_tests.py b/tests/run_tests.py
index 664ebcc2..a30a0391 100755
--- a/tests/run_tests.py
+++ b/tests/run_tests.py
@@ -5,10 +5,8 @@ import argparse
 import logging
 
 import plumbum
-from plumbum.cmd import pytest
-
 from images_hierarchy import get_test_dirs
-
+from plumbum.cmd import pytest
 
 LOGGER = logging.getLogger(__name__)
 
diff --git a/tests/scipy-notebook/data/cython/setup.py b/tests/scipy-notebook/data/cython/setup.py
index de123d62..2ad2140c 100644
--- a/tests/scipy-notebook/data/cython/setup.py
+++ b/tests/scipy-notebook/data/cython/setup.py
@@ -1,4 +1,6 @@
-from setuptools import setup
-from Cython.Build import cythonize
+# These lines are not sorted by isort on purpose
+# see: https://stackoverflow.com/a/53356077/4881441
+from setuptools import setup  # isort:skip
+from Cython.Build import cythonize  # isort:skip
 
 setup(ext_modules=cythonize("helloworld.pyx"))
diff --git a/tests/scipy-notebook/data/matplotlib/matplotlib_1.py b/tests/scipy-notebook/data/matplotlib/matplotlib_1.py
index 11b10dd3..e1a0add6 100644
--- a/tests/scipy-notebook/data/matplotlib/matplotlib_1.py
+++ b/tests/scipy-notebook/data/matplotlib/matplotlib_1.py
@@ -1,11 +1,12 @@
 # Matplotlib: Create a simple plot example.
 # Refs: https://matplotlib.org/stable/gallery/lines_bars_and_markers/simple_plot.html
 
+import os
+
 # Optional test with [Matplotlib Jupyter Integration](https://github.com/matplotlib/ipympl)
 # %matplotlib widget
 import matplotlib.pyplot as plt
 import numpy as np
-import os
 
 # Data for plotting
 t = np.arange(0.0, 2.0, 0.01)
diff --git a/tests/scipy-notebook/data/matplotlib/matplotlib_fonts_1.py b/tests/scipy-notebook/data/matplotlib/matplotlib_fonts_1.py
index afc50fb3..951a3d87 100644
--- a/tests/scipy-notebook/data/matplotlib/matplotlib_fonts_1.py
+++ b/tests/scipy-notebook/data/matplotlib/matplotlib_fonts_1.py
@@ -1,7 +1,8 @@
 # Matplotlib: Test tex fonts
+import os
+
 import matplotlib
 import matplotlib.pyplot as plt
-import os
 
 matplotlib.rcParams["pgf.texsystem"] = "pdflatex"
 matplotlib.rcParams.update(
diff --git a/tests/scipy-notebook/test_extensions.py b/tests/scipy-notebook/test_extensions.py
index 3f457cad..ca2a5f4e 100644
--- a/tests/scipy-notebook/test_extensions.py
+++ b/tests/scipy-notebook/test_extensions.py
@@ -3,7 +3,6 @@
 import logging
 
 import pytest  # type: ignore
-
 from conftest import TrackedContainer
 
 LOGGER = logging.getLogger(__name__)
diff --git a/tests/scipy-notebook/test_matplotlib.py b/tests/scipy-notebook/test_matplotlib.py
index bf5aacbc..725f8a9e 100644
--- a/tests/scipy-notebook/test_matplotlib.py
+++ b/tests/scipy-notebook/test_matplotlib.py
@@ -2,10 +2,9 @@
 # Distributed under the terms of the Modified BSD License.
 
 import logging
-
-import pytest  # type: ignore
 from pathlib import Path
 
+import pytest  # type: ignore
 from conftest import TrackedContainer
 
 LOGGER = logging.getLogger(__name__)
diff --git a/tests/scipy-notebook/units/unit_pandas.py b/tests/scipy-notebook/units/unit_pandas.py
index 5036f823..fc998225 100644
--- a/tests/scipy-notebook/units/unit_pandas.py
+++ b/tests/scipy-notebook/units/unit_pandas.py
@@ -4,6 +4,5 @@
 import numpy as np
 import pandas as pd
 
-
 np.random.seed(0)
 print(pd.Series(np.random.randint(0, 7, size=10)).sum())
diff --git a/tests/tensorflow-notebook/units/unit_tensorflow.py b/tests/tensorflow-notebook/units/unit_tensorflow.py
index 0f5911ca..96446a5d 100644
--- a/tests/tensorflow-notebook/units/unit_tensorflow.py
+++ b/tests/tensorflow-notebook/units/unit_tensorflow.py
@@ -2,6 +2,5 @@
 # Distributed under the terms of the Modified BSD License.
 import tensorflow as tf
 
-
 print(tf.constant("Hello, TensorFlow"))
 print(tf.reduce_sum(tf.random.normal([1000, 1000])))